Protocol for nuclear dissociation of the adult C. elegans for single-nucleus RNA sequencing and its application for mapping environmental responses.
STAR Protoc
; 4(4): 102756, 2023 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-38043054
ABSTRACT
Caenorhabditis elegans is a valuable model to study organ, tissue, and cell-type responses to external cues. However, the nematode comprises multiple syncytial tissues with spatial coordinates corresponding to distinct nuclear transcriptomes. Here, we present a single-nucleus RNA sequencing (snRNA-seq) protocol that aims to overcome difficulties encountered with single-cell RNA sequencing in C. elegans. We describe steps for isolating C. elegans nuclei for downstream applications including snRNA-seq applied to the context of alcohol exposure. For complete details on the use and execution of this protocol, please refer to Truong et al. (2023).1.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Núcleo Celular
/
Caenorhabditis elegans
Límite:
Animals
Idioma:
En
Año:
2023
Tipo del documento:
Article