High-capacity sample multiplexing for single cell chromatin accessibility profiling.
BMC Genomics
; 24(1): 737, 2023 Dec 04.
Article
en En
| MEDLINE
| ID: mdl-38049719
ABSTRACT
Single-cell chromatin accessibility has emerged as a powerful means of understanding the epigenetic landscape of diverse tissues and cell types, but profiling cells from many independent specimens is challenging and costly. Here we describe a novel approach, sciPlex-ATAC-seq, which uses unmodified DNA oligos as sample-specific nuclear labels, enabling the concurrent profiling of chromatin accessibility within single nuclei from virtually unlimited specimens or experimental conditions. We first demonstrate our method with a chemical epigenomics screen, in which we identify drug-altered distal regulatory sites predictive of compound- and dose-dependent effects on transcription. We then analyze cell type-specific chromatin changes in PBMCs from multiple donors responding to synthetic and allogeneic immune stimulation. We quantify stimulation-altered immune cell compositions and isolate the unique effects of allogeneic stimulation on chromatin accessibility specific to T-lymphocytes. Finally, we observe that impaired global chromatin decondensation often coincides with chemical inhibition of allogeneic T-cell activation.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ADN
/
Cromatina
Idioma:
En
Año:
2023
Tipo del documento:
Article