Transcriptome analysis reveals the underlying mechanism for over-accumulation of alkaline protease in Bacillus licheniformis.
J Appl Microbiol
; 135(2)2024 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-38159929
ABSTRACT
AIMS:
Bacillus licheniformis AQ is an industrial strain with high production of alkaline protease (AprE), which has great industrial application value. However, how to regulate the production of AprE in the process of industrial fermentation is still not completely clear. Therefore, it is important to understand the metabolic process of AprE production in the industrial fermentation medium. METHODS ANDRESULTS:
In this study, transcriptome sequencing of the whole fermentation course was performed to explore the synthesis and regulation mechanism of AprE in B. licheniformis AQ. During the fermentation process, the AprE got continuously accumulated, reaching a peak of 42 020 U/mL at the fermentation endpoint (48 h). Meanwhile, the highly expressed genes were observed. Compared with the fermentation endpoint, there were 61 genes in the intersection of differentially expressed genes, functioning as catabolic processes, peptidases and inhibitors, chaperones, and folding catalysts. Furthermore, the protein-protein interactions network of AprE was constructed.CONCLUSION:
This study provides important transcriptome information for B. licheniformis AQ and potential molecular targets for further improving the production of AprE.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Bacillus licheniformis
Idioma:
En
Año:
2024
Tipo del documento:
Article