Microbacterium memoriense sp. nov., a member of the Actinomycetota from marine beach sediment of the north coast of Portugal.
Int J Syst Evol Microbiol
; 74(1)2024 Jan.
Article
en En
| MEDLINE
| ID: mdl-38189368
ABSTRACT
The oceans harbour a myriad of unknown micro-organisms that remain unstudied because of a failure to establish the right growth conditions under laboratory conditions. To overcome this limitation, an isolation effort inspired by the iChip was performed using marine sediments from Memória beach, Portugal. A novel strain, PMIC_1C1BT, was obtained and subjected to a polyphasic study. Cells of strain PMIC_1C1BT were Gram-positive, rod-shaped, divided by binary fission and formed colonies that were shiny light-yellow. Based on its full 16S rRNA gene sequence, strain PMIC_1C1BT was phylogenetically associated to the genus Microbacterium and its closest relatives were Microbacterium aurum KACC 15219T (98.55â%), Microbacterium diaminobutyricum RZ63T (98.48â%) and Microbacterium hatanonis JCM 14558T (98.13â%). Strain PMIC_1C1BT had a genome size of 2â761â607 bp with 67.71âmol% of G+C content and 2582 coding sequences, which is lower than the genus average. Strain PMIC_1C1BT grew from 15 to 30â°C, optimally at 25â°C, at pH 6.0 to 11.0, optimally between pH 6.0 and 8.0, and from 0 to 5â% (w/v) NaCl, optimally between 2.0 and 3.0â%. It grew with casamino acids, glutamine, methionine, N-acetylglucosamine, sodium nitrate, tryptophan, urea and valine as sole nitrogen sources, and arabinose and cellobiose as sole carbon sources. The major cellular fatty acids were anteiso-C15ââ0, iso-C16ââ0 and iso-C17ââ0. Genome mining revealed the presence of four biosynthetic gene clusters (BGCs) with low similarities to other known BCGs. Based on the polyphasic data, strain PMIC_1C1BT is proposed to represent a novel species, for which the name Microbacterium memoriense sp. nov. (=CECT 30366T=LMG 32350T) is proposed.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Actinomycetales
/
Microbacterium
País como asunto:
Europa
Idioma:
En
Año:
2024
Tipo del documento:
Article