A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus.
Cell Biol Int
; 48(3): 347-357, 2024 Mar.
Article
en En
| MEDLINE
| ID: mdl-38212941
ABSTRACT
Cell lines are important in vitro models to answer biological mechanisms with less genetic variations. The present study was attempted to develop a cell line from rainbow trout, where we obtained a cell line from the heart, named "RBT-H." The cell line was authenticated using karyotyping and cytochrome c oxidase subunit I (COI) gene sequencing. The karyotype demonstrated diploid chromosome number (2n) as 62 and the sequence of partial COI gene was 99.84% similar to rainbow trout COI data set, both suggesting the origin of RBT-H from the rainbow trout. The heart cell line was mycoplasma-free and found to be refractory to infection with the Tilapia lake virus. The RBT-H cell line is deposited in the National Repository of Fish Cell Line (NRFC) at ICAR-NBFGR, Lucknow, India, with Accession no. NRFC0075 for maintenance and distribution to researchers on request for R&D.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tilapia
/
Oncorhynchus mykiss
/
Enfermedades de los Peces
Límite:
Animals
País como asunto:
Asia
Idioma:
En
Año:
2024
Tipo del documento:
Article