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Molecular mechanism of anticancer effect of heat shock protein 90 inhibitor BIIB021 in human bladder cancer cell line.
Asdemir, Aydemir; Özgür, Aykut.
  • Asdemir A; Faculty of Medicine, Department of Urology, Sivas Cumhuriyet University, Sivas, Turkey. aydemirasdemir@gmail.com.
  • Özgür A; Artova Vocational School, Department of Veterinary Medicine, Laboratory and Veterinary Health Program, Tokat Gaziosmanpasa University, Tokat, Turkey.
Naunyn Schmiedebergs Arch Pharmacol ; 397(7): 5167-5177, 2024 07.
Article en En | MEDLINE | ID: mdl-38240781
ABSTRACT
Bladder cancer is a type of urologic malignancy that exhibits significant morbidity, mortality, and treatment costs. Inhibition of heat shock protein 90 (HSP90) activity has been a promising pharmacological strategy for blocking of bladder cancer pathogenesis. BIIB021 is a next-generation HSP90 inhibitor which interrupts ATP hydrolysis process of HSP90 and inhibits the stabilization and correct folding of client proteins. In current study, we aimed to investigate the molecular mechanism of the anticancer activity of BIIB021 in human bladder cancer T24 cells. Our results revealed that nanomolar concentration of BIIB021 decreased viability of T24 cell. BIIB021 downregulated HSP90 expression in T24 cells and inhibited the refolding activity of luciferase in the presence of T24 cell lysate. PCR array data indicated a significant alteration in transcript levels of cancer-related genes involved in metastases, apoptotic cell death, cell cycle, cellular senescence, DNA damage and repair mechanisms, epithelial-to-mesenchymal transition, hypoxia, telomeres and telomerase, and cancer metabolism pathways in T24 cells. All findings hypothesize that BIIB021 could exhibit as effective HSP90 inhibitor in the future for treatment of bladder cancer patients.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Proteínas HSP90 de Choque Térmico / Antineoplásicos Límite: Humans Idioma: En Año: 2024 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Proteínas HSP90 de Choque Térmico / Antineoplásicos Límite: Humans Idioma: En Año: 2024 Tipo del documento: Article