Volatile Organic Compounds from Offspring of Stingless Bee Sacrificed in Hygienic Behavior Test.
Chem Biodivers
; 21(3): e202301641, 2024 Mar.
Article
en En
| MEDLINE
| ID: mdl-38358043
ABSTRACT
This study shows the profile of volatile organic compounds (VOCs) from pupae and larvae of Melipona quadrifasciata anthidioides Lepeletier subjected to three death induction techniques for hygienic behavior (HB) studies freezing in liquid nitrogen (LN2), freezing in a freezer (FRZ) and piercing of offspring with an entomological pin (PIN). The VOCs from larvae and pupae were obtained through headspace solid-phase microextraction and characterized using gas chromatography coupled to mass spectrometry. In addition, an HB test was performed on the colonies. The main classes of VOCs were hydrocarbons, terpenes and alcohols. Multivariate analysis was applied and showed that there was a separation in the compound profiles between the different treatments. The HB test in the colonies showed that 24â
hours after the application of the techniques, the bees removed more dead larvae in LN2 treatment (83.5 %), while after 48â
hours more larvae were removed in the LN2 and FRZ treatments (92.3 %). When compared to pupae removal, larvae removal was significantly faster in LN2.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Compuestos Orgánicos Volátiles
Límite:
Animals
Idioma:
En
Año:
2024
Tipo del documento:
Article