Dosage-effect of selenium supplementation on blood glucose and oxidative stress in type 2 diabetes mellitus and normal mice.
J Trace Elem Med Biol
; 83: 127410, 2024 May.
Article
en En
| MEDLINE
| ID: mdl-38377660
ABSTRACT
BACKGROUND:
The effectiveness of selenium (Se) supplementation on glycemic control is disparate.OBJECTIVE:
This study aims to evaluate the effects of different dosages of Se diets on the blood glucose in type 2 diabetes mellitus (T2DM, db/db) and normal (db/m) mice.METHODS:
The db/db and db/m mice were fed with different dosages of Se supplemented diets (0, 0.1, 0.3, 0.9, 2.7 mg/kg) for 12 weeks, respectively. Se concentrations of tissues, physical and biochemical characteristics, oxidative stress indexes and gene expression related to glucose, lipid metabolism and Se transporters of liver were detected.RESULTS:
The Se concentrations in tissues were related to the dosages of Se supplementation in db/db (blood slope=11.69, r = 0.924; skeletal muscle slope=0.36, r = 0.505; liver slope=22.12, r = 0.828; kidney slope=11.81, r = 0.736) and db/m mice (blood slope=19.89, r = 0.876; skeletal muscle slope=2.80, r = 0.883; liver slope=44.75, r = 0.717; kidney slope=60.15, r = 0.960). Compared with Se2.7 group, the fasting blood glucose (FBG) levels of Se0.1 and Se0.3 group were decreased at week3 in db/db mice. Compared with control (Se0) group, the FBG levels of Se2.7 group were increased from week6 to week12 in db/m mice. The oral glucose tolerance test (OGTT) showed that the area under the curve (AUC) of Se0.3 group was lower than that of Se0.9 and Se2.7 group in db/m mice. Furthermore, compared with control group, the malondialdehyde (MDA) level in skeletal muscle of Se0.1 group was decreased, while that of Se2.7 group was increased in db/db mice; the glutathione peroxidase (GPx) activity in skeletal muscle of Se0.3, Se0.9 and Se2.7 group was increased both in db/db and db/m mice. For db/db mice, glucose-6-phosphatase catalytic (G6pc) expression of other groups were lower and fatty acid synthase (Fasn) expression of Se0.9 group were lower compared with Se0.3 group. For db/m mice, compared with Se0.3 group, (peroxisome proliferative activated receptor gamma coactivator 1 alpha) Pgc-1α expression of control and Se0.9 group were higher; (phosphoenolpyruvate carboxykinase 1) Pck1 expression of Se0.1, Se0.9, and Se2.7 group were higher.CONCLUSION:
Low dosages (0.1 and 0.3 mg/kg) of Se supplementation exerted beneficial effects on FBG levels and glucose tolerance through regulating hepatic glycolysis and gluconeogenesis and inhibit the oxidative stress while high dosages of Se (0.9 and 2.7 mg/kg) supplementation enhanced FBG levels, impaired glucose tolerance and aggravate oxidative stress.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Selenio
/
Diabetes Mellitus Tipo 2
Límite:
Animals
Idioma:
En
Año:
2024
Tipo del documento:
Article