A Benchtop Round Window Model for Studying Magnetic Nanoparticle Transport to the Inner Ear.
Laryngoscope
; 134(7): 3355-3362, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38379206
ABSTRACT
INTRODUCTION:
The round window membrane (RWM) presents a significant barrier to the local application of therapeutics to the inner ear. We demonstrate a benchtop preclinical RWM model and evaluate superparamagnetic iron oxide nanoparticles (SPIONs) as vehicles for magnetically assisted drug delivery.METHODS:
Guinea pig RWM explants were inset into a 3D-printed dual chamber benchtop device. Custom-synthesized 7-nm iron core nanoparticles were modified with different polyethylene glycol chains to yield two sizes of SPIONs (NP-PEG600 and NP-PEG3000) and applied to the benchtop model with and without a magnetic field. Histologic analysis of the RWM was performed using transmission electron microscopy (TEM) and confocal microscopy.RESULTS:
Over a 4-h period, 19.5 ± 1.9% of NP-PEG3000 and 14.6 ± 1.9% of NP-PEG600 were transported across the guinea pig RWM. The overall transport increased by 1.45× to 28.4 ± 5.8% and 21.0 ± 2.0%, respectively, when a magnetic field was applied. Paraformaldehyde fixation of the RWM decreased transport significantly (NP-PEG3000 7.6 ± 1.5%; NP-PEG600 7.0 ± 1.6%). Confocal and electron microscopy analysis demonstrated nanoparticle localization throughout all cellular layers and layer-specific transport characteristics within RWM.CONCLUSION:
The guinea pig RWM explant benchtop model allows for targeted and practical investigations of transmembrane transport in the development of nanoparticle drug delivery vehicles. The presence of a magnetic field increases SPION delivery by 45%-50% in a nanoparticle size- and cellular layer-dependent manner. LEVEL OF EVIDENCE NA Laryngoscope, 1343355-3362, 2024.Palabras clave
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Banco de datos:
MEDLINE
Asunto principal:
Ventana Redonda
/
Sistemas de Liberación de Medicamentos
Límite:
Animals
Idioma:
En
Año:
2024
Tipo del documento:
Article