[Thioredoxin interacting protein in fibroblasts of human dermis in the process of aging.]

ABSTRACT

The aim of this work was to examine the content of thioredoxin interacting protein in fibroblasts of human dermis from the development until 85 years old, and defining of a role of thioredoxin interacting protein in age-dependent changes in the number of fibroblasts in the dermis. Thioredoxin interacting protein, proliferating cells nuclear antigen (PCNA), marker of fibroblasts vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for thioredoxin interacting protein in the dermis is increased from 20 weeks of pregnancy until 60 years old followed by a little decrease in age interval 61-85 years old. General number and percent of PCNA positive fibroblasts in dermis are decreased with age with more expressed changes until 40 years old. Correlation analysis showed that age dependent decrease in the number of fibroblasts and their proliferative activity is significantly associated with increase in thioredoxin interacting protein positive fibroblasts in dermis. Results obtained allow to suggest that thioredoxin interacting protein plays a role in age dependent decrease in the number of fibroblasts and their proliferation in human dermis.