Your browser doesn't support javascript.
loading
Histoplasty Modification of the Tumor Microenvironment in a Murine Preclinical Model of Breast Cancer.
Pieper, Alexander A; Stowe, Nicholas A; Periyasamy, Sarvesh; Burkel, Brian M; Tsarovsky, Noah W; Singh, Ajay P; Rakhmilevich, Alexander L; Sondel, Paul M; Ponik, Suzanne M; Laeseke, Paul F; Yu, John-Paul J.
  • Pieper AA; Department of Radiology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin; Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
  • Stowe NA; Department of Radiology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
  • Periyasamy S; Department of Medical Physics, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
  • Burkel BM; Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, Wisconsin.
  • Tsarovsky NW; Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
  • Singh AP; Graduate Program in Cellular and Molecular Biology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
  • Rakhmilevich AL; Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
  • Sondel PM; Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin; Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.
  • Ponik SM; Department of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, Wisconsin.
  • Laeseke PF; Department of Radiology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin; Department of Medical Physics, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison,
  • Yu JJ; Department of Radiology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin; Department of Psychiatry, University of Wisconsin-Madison, Madison, Wisconsin; Graduate Program in Cellular and Molecular Biology, University of Wisconsin School of Medicine and Public Health,
J Vasc Interv Radiol ; 35(6): 900-908.e2, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38508448
ABSTRACT

PURPOSE:

To develop a noninvasive therapeutic approach able to alter the biophysical organization and physiology of the extracellular matrix (ECM) in breast cancer. MATERIALS AND

METHODS:

In a 4T1 murine model of breast cancer, histoplasty treatment with a proprietary 700-kHz multielement therapy transducer using a coaxially aligned ultrasound (US) imaging probe was used to target the center of an ex vivo tumor and deliver subablative acoustic energy. Tumor collagen morphology was qualitatively evaluated before and after histoplasty with second harmonic generation. Separately, mice bearing bilateral 4T1 tumors (n = 4; total tumors = 8) were intravenously injected with liposomal doxorubicin. The right flank tumor was histoplasty-treated, and tumors were fluorescently imaged to detect doxorubicin uptake after histoplasty treatment. Next, 4T1 tumor-bearing mice were randomized into 2 treatment groups (sham vs histoplasty, n = 3 per group). Forty-eight hours after sham/histoplasty treatment, tumors were harvested and analyzed using flow cytometry.

RESULTS:

Histoplasty significantly increased (P = .002) liposomal doxorubicin diffusion into 4T1 tumors compared with untreated tumors (2.12- vs 1.66-fold increase over control). Flow cytometry on histoplasty-treated tumors (n = 3) demonstrated a significant increase in tumor macrophage frequency (42% of CD45 vs 33%; P = .022) and a significant decrease in myeloid-derived suppressive cell frequency (7.1% of CD45 vs 10.3%; P = .044). Histoplasty-treated tumors demonstrated increased CD8+ (5.1% of CD45 vs 3.1%; P = .117) and CD4+ (14.1% of CD45 vs 11.8%; P = .075) T-cell frequency.

CONCLUSIONS:

Histoplasty is a nonablative focused US approach to noninvasively modify the tumor ECM, increase chemotherapeutic uptake, and alter the tumor immune microenvironment.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Doxorrubicina / Microambiente Tumoral / Ratones Endogámicos BALB C Límite: Animals Idioma: En Año: 2024 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Doxorrubicina / Microambiente Tumoral / Ratones Endogámicos BALB C Límite: Animals Idioma: En Año: 2024 Tipo del documento: Article