Cylindrospermopsin enhances the conjugative transfer of plasmid-mediated multi-antibiotic resistance genes through glutathione biosynthesis inhibition.
Ecotoxicol Environ Saf
; 276: 116288, 2024 May.
Article
en En
| MEDLINE
| ID: mdl-38581909
ABSTRACT
Cylindrospermopsin (CYN), a cyanobacterial toxin, has been detected in the global water environment. However, information concerning the potential environmental risk of CYN is limited, since the majority of previous studies have mainly focused on the adverse health effects of CYN through contaminated drinking water. The present study reported that CYN at environmentally relevant levels (0.1-100⯵g/L) can significantly enhance the conjugative transfer of RP4 plasmid in Escherichia coli genera, wherein application of 10⯵g/L of CYN led to maximum fold change of â¼6.5- fold at 16â¯h of exposure. Meanwhile, evaluation of underlying mechanisms revealed that environmental concentration of CYN exposure could increase oxidative stress in the bacterial cells, resulting in ROS overproduction. In turn, this led to an upregulation of antioxidant enzyme-related genes to avoid ROS attack. Further, inhibition of the synthesis of glutathione (GSH) was also detected, which led to the rapid depletion of GSH in cells and thus triggered the SOS response and promoted the conjugative transfer process. Increase in cell membrane permeability, upregulation of expression of genes related to pilus generation, ATP synthesis, and RP4 gene expression were also observed. These results highlight the potential impact on the spread of antimicrobial resistance in water environments.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Plásmidos
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Toxinas Bacterianas
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Uracilo
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Alcaloides
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Escherichia coli
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Toxinas de Cianobacterias
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Glutatión
Idioma:
En
Año:
2024
Tipo del documento:
Article