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Multi-omics study reveals different pathogenesis of the generation of skin lesions in SLE and IDLE patients.
Li, Qianwen; Jia, Chen; Pan, Wenjing; Liu, Hongmei; Tang, Congli; Weber, Daniel; Chen, Kaili; Long, Hai; Byrne-Steele, Miranda L; Han, Jian; He, Nongyue; Xiao, Rong; Zhao, Ming; Che, Nan; Guo, Qing; Gui, Guangji; Li, Shanshan; Si, Henan; Guo, Shuping; Liu, Hongye; Wang, Gang; Zhu, Guannan; Yang, Bin; Wang, Yu; Ding, Yan; Yang, Xianxu; Akihiko, Yoshimura; Lu, Liwei; Chang, Christopher; Chan, Vera; Lau, Chak-Sing; Qi, Hai; Liu, Wanli; Li, Song; Wu, Haijing; Lu, Qianjin.
  • Li Q; Department of Dermatology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, 410011, China.
  • Jia C; Department of Dermatology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, 410011, China.
  • Pan W; Nanjing ARP Biotechnology Co., Ltd, Nanjing, Jiangsu, China; iRepertoire Inc., Huntsville, AL, USA.
  • Liu H; Hunan University of Technology, Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Zhuzhou, Hunan, China.
  • Tang C; Nanjing ARP Biotechnology Co., Ltd, Nanjing, Jiangsu, China.
  • Weber D; iRepertoire Inc., Huntsville, AL, USA.
  • Chen K; Department of Dermatology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, 410011, China.
  • Long H; Department of Dermatology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, 410011, China.
  • Byrne-Steele ML; iRepertoire Inc., Huntsville, AL, USA.
  • Han J; iRepertoire Inc., Huntsville, AL, USA.
  • He N; Nanjing ARP Biotechnology Co., Ltd, Nanjing, Jiangsu, China.
  • Xiao R; Department of Dermatology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, 410011, China.
  • Zhao M; Department of Dermatology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, 410011, China; Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu, 210042, China; Key Labora
  • Che N; Department of Rheumatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
  • Guo Q; Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510000, China.
  • Gui G; Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510000, China.
  • Li S; Department of Dermatology, The First Bethune Hospital of Jilin University, Changchun, Jilin, 130000, China.
  • Si H; Department of Dermatology, The First Bethune Hospital of Jilin University, Changchun, Jilin, 130000, China.
  • Guo S; Department of Dermatology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030000, China.
  • Liu H; Department of Dermatology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030000, China.
  • Wang G; Department of Dermatology, Xijing Hospital, Xi'an, Shaanxi, 710000, China.
  • Zhu G; Department of Dermatology, Xijing Hospital, Xi'an, Shaanxi, 710000, China.
  • Yang B; Dermatology Hospital of Southern Medical University, Guangzhou, Guangdong, 510000, China.
  • Wang Y; Dermatology Hospital of Southern Medical University, Guangzhou, Guangdong, 510000, China.
  • Ding Y; Hainan Provincial Hospital of Skin Disease, Haikou, Hainan, 570100, China.
  • Yang X; Hainan Provincial Hospital of Skin Disease, Haikou, Hainan, 570100, China.
  • Akihiko Y; Department of Microbiology and Immunology, Keio University School of Medicine 35 Shinanoomachi, Shinjyuku-ku, Tokyo, 160-8582, Japan.
  • Lu L; Department of Pathology and Shenzhen Institute of Research and Innovation, The University of Hong Kong, Hong Kong, 999077, China.
  • Chang C; Division of Immunology, Allergy and Rheumatology, Memorial Healthcare System, Joe DiMaggio Children's Hospital, Hollywood, FL, USA.
  • Chan V; Division of Rheumatology & Clinical Immunology, Department of Medicine, University of Hong Kong, Hong Kong, China.
  • Lau CS; Division of Rheumatology & Clinical Immunology, Department of Medicine, University of Hong Kong, Hong Kong, China.
  • Qi H; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
  • Liu W; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
  • Li S; Hunan University of Technology, Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Zhuzhou, Hunan, China. Electronic address: sosong1980@gmail.com.
  • Wu H; Department of Dermatology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, 410011, China. Electronic address: chriswu1010@csu.edu.cn.
  • Lu Q; Department of Dermatology, The Second Xiangya Hospital of Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, 410011, China; Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu, 210042, China; Key Labora
J Autoimmun ; 146: 103203, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38643729
ABSTRACT
Lupus erythematosus (LE) is a heterogeneous, antibody-mediated autoimmune disease. Isolate discoid LE (IDLE) and systematic LE (SLE) are traditionally regarded as the two ends of the spectrum, ranging from skin-limited damage to life-threatening multi-organ involvement. Both belong to LE, but IDLE and SLE differ in appearance of skin lesions, autoantibody panels, pathological changes, treatments, and immunopathogenesis. Is discoid lupus truly a form of LE or is it a completely separate entity? This question has not been fully elucidated. We compared the clinical data of IDLE and SLE from our center, applied multi-omics technology, such as immune repertoire sequencing, high-resolution HLA alleles sequencing and multi-spectrum pathological system to explore cellular and molecular phenotypes in skin and peripheral blood from LE patients. Based on the data from 136 LE patients from 8 hospitals in China, we observed higher damage scores and fewer LE specific autoantibodies in IDLE than SLE patients, more uCDR3 sharing between PBMCs and skin lesion from SLE than IDLE patients, elevated diversity of V-J recombination in IDLE skin lesion and SLE PBMCs, increased SHM frequency and class switch ratio in IDLE skin lesion, decreased SHM frequency but increased class switch ratio in SLE PBMCs, HLA-DRB1*03010101, HLA-B*58010101, HLA-C*03020201, and HLA-DQB1*02010101 positively associated with SLE patients, and expanded Tfh-like cells with ectopic germinal center structures in IDLE skin lesions. These findings suggest a significant difference in the immunopathogenesis of skin lesions between SLE and IDLE patients. SLE is a B cell-predominate systemic immune disorder, while IDLE appears limited to the skin. Our findings provide novel insights into the pathogenesis of IDLE and other types of LE, which may direct more accurate diagnosis and novel therapeutic strategies.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Piel / Autoanticuerpos / Lupus Eritematoso Discoide / Lupus Eritematoso Sistémico Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Año: 2024 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Piel / Autoanticuerpos / Lupus Eritematoso Discoide / Lupus Eritematoso Sistémico Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Año: 2024 Tipo del documento: Article