Cytotoxic effects of aporphine alkaloids from the stems and leaves of Stephania dielsiana Y.C.Wu.
Nat Prod Res
; 38(11): 1864-1873, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38739563
ABSTRACT
Phytochemical studies of the stems and leaves of Stephania dielsiana Y.C.Wu yielded two new aporphine alkaloids (1 and 5), along with six known alkaloids (2-4 and 6-8). Their structures were characterised based on analyses of spectroscopic data, including one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy and high-resolution electrospray ionisation mass spectrometry (HR-ESI-MS). The cytotoxic activities of the isolated compounds against a small panel of tumour cell lines were assessed by MTS assay. Interestingly, compound 2 exhibited particularly strong cytotoxic activities against HepG2, MCF7 and OVCAR8 cancer cell lines, with IC50 values of 3.20 ± 0.18, 3.10 ± 0.06 and 3.40 ± 0.007 µM, respectively. Furthermore, molecular docking simulations were carried out to explore the interactions and binding mechanisms of the most active compound (compound 2) with proteins. Our results contribute to understanding the secondary metabolites produced by S. dielsiana and provide a scientific rationale for further investigations of cytotoxicity of this valuable medicinal plant.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Aporfinas
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Tallos de la Planta
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Hojas de la Planta
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Stephania
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Alcaloides
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Simulación del Acoplamiento Molecular
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Antineoplásicos Fitogénicos
Límite:
Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article