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Aberrant expression of NEDD4L disrupts mitochondrial homeostasis by downregulating CaMKKß in diabetic kidney disease.
Han, Fei; Wu, Shi; Dong, Ya; Liu, Yanjie; Sun, Bei; Chen, Liming.
  • Han F; NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital Tianjin and Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, China.
  • Wu S; NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital Tianjin and Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, China.
  • Dong Y; NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital Tianjin and Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, China.
  • Liu Y; NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital Tianjin and Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, China.
  • Sun B; NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital Tianjin and Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, China.
  • Chen L; NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital Tianjin and Institute of Endocrinology, Tianjin Medical University, Tianjin, 300134, China. xfx22081@vip.163.com.
J Transl Med ; 22(1): 465, 2024 May 16.
Article en En | MEDLINE | ID: mdl-38755664
ABSTRACT
Disturbance in mitochondrial homeostasis within proximal tubules is a critical characteristic associated with diabetic kidney disease (DKD). CaMKKß/AMPK signaling plays an important role in regulating mitochondrial homeostasis. Despite the downregulation of CaMKKß in DKD pathology, the underlying mechanism remains elusive. The expression of NEDD4L, which is primarily localized to renal proximal tubules, is significantly upregulated in the renal tubules of mice with DKD. Coimmunoprecipitation (Co-IP) assays revealed a physical interaction between NEDD4L and CaMKKß. Moreover, deletion of NEDD4L under high glucose conditions prevented rapid CaMKKß protein degradation. In vitro studies revealed that the aberrant expression of NEDD4L negatively influences the protein stability of CaMKKß. This study also explored the role of NEDD4L in DKD by using AAV-shNedd4L in db/db mice. These findings confirmed that NEDD4L inhibition leads to a decrease in urine protein excretion, tubulointerstitial fibrosis, and oxidative stress, and mitochondrial dysfunction. Further in vitro studies demonstrated that si-Nedd4L suppressed mitochondrial fission and reactive oxygen species (ROS) production, effects antagonized by si-CaMKKß. In summary, the findings provided herein provide strong evidence that dysregulated NEDD4L disturbs mitochondrial homeostasis by negatively modulating CaMKKß in the context of DKD. This evidence underscores the potential of therapeutic interventions targeting NEDD4L and CaMKKß to safeguard renal tubular function in the management of DKD.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Regulación hacia Abajo / Nefropatías Diabéticas / Quinasa de la Proteína Quinasa Dependiente de Calcio-Calmodulina / Ubiquitina-Proteína Ligasas Nedd4 / Homeostasis / Mitocondrias Límite: Animals / Humans / Male Idioma: En Año: 2024 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Regulación hacia Abajo / Nefropatías Diabéticas / Quinasa de la Proteína Quinasa Dependiente de Calcio-Calmodulina / Ubiquitina-Proteína Ligasas Nedd4 / Homeostasis / Mitocondrias Límite: Animals / Humans / Male Idioma: En Año: 2024 Tipo del documento: Article