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Nanopore Blockade Sensors for Quantitative Analysis Using an Optical Nanopore Assay.
Doan, Thanh Hoang Phuong; Fried, Jasper P; Tang, Wenxian; Hagness, Daniel Everett; Yang, Ying; Wu, Yanfang; Tilley, Richard D; Gooding, J Justin.
  • Doan THP; School of Chemistry, Australian Centre for NanoMedicine, The University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Fried JP; School of Chemistry, Australian Centre for NanoMedicine, The University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Tang W; School of Chemistry, Australian Centre for NanoMedicine, The University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Hagness DE; School of Chemistry, Australian Centre for NanoMedicine, The University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Yang Y; School of Chemistry, Australian Centre for NanoMedicine, The University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Wu Y; School of Chemistry, Australian Centre for NanoMedicine, The University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Tilley RD; School of Chemistry, Australian Centre for NanoMedicine, The University of New South Wales, Sydney, New South Wales 2052, Australia.
  • Gooding JJ; Electron Microscope Unit, Mark Wainwright Analytical Centre, The University of New South Wales, Sydney, New South Wales 2052, Australia.
Nano Lett ; 24(21): 6218-6224, 2024 May 29.
Article en En | MEDLINE | ID: mdl-38757765
ABSTRACT
Nanopore sensing is a popular biosensing strategy that is being explored for the quantitative analysis of biomarkers. With low concentrations of analytes, nanopore sensors face challenges related to slow response times and selectivity. Here, we demonstrate an approach to rapidly detect species at ultralow concentrations using an optical nanopore blockade sensor for quantitative detection of the protein vascular endothelial growth factor (VEGF). This sensor relies on monitoring fluorescent polystyrene nanoparticles blocking nanopores in a nanopore array of 676 nanopores. The fluorescent signal is read out using a wide-field fluorescence microscope. Nonspecific blockade events are then distinguished from specific blockade events based on the ability to pull the particles out of the pore using an applied electric field. This allows the detection of VEGF at sub-picomolar concentration in less than 15 min.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Poliestirenos / Técnicas Biosensibles / Factor A de Crecimiento Endotelial Vascular / Nanoporos Límite: Humans Idioma: En Año: 2024 Tipo del documento: Article
Texto completo
Imprimir
XML
PubMed Links
Search on Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Poliestirenos / Técnicas Biosensibles / Factor A de Crecimiento Endotelial Vascular / Nanoporos Límite: Humans Idioma: En Año: 2024 Tipo del documento: Article