Overexpression of miR-96 leads to retinal degeneration in mice.
Biochem Biophys Res Commun
; 719: 150048, 2024 Jul 30.
Article
en En
| MEDLINE
| ID: mdl-38763044
ABSTRACT
Double knockout of miR-183 and miR-96 results in retinal degeneration in mice; however, single knockout of miR-96 leads to developmental delay but not substantial retinal degeneration. To further explore the role of miR-96, we overexpressed this miRNA in mouse retinas. Interestingly, we found that overexpression of miR-96 at a safe dose results in retinal degeneration in the mouse retina. The retinal photoreceptors dramatically degenerated in the miR-96-overexpressing group, as shown by OCT, ERG and cryosectioning at one month after subretinal injection. Degenerative features such as TUNEL signals and reactive gliosis were observed in the miR-96-overexpressing retina. RNA-seq data revealed that immune responses and microglial activation occurred in the degenerating retina. Further qRTâPCR and immunostaining experiments verified the microglial activation. Moreover, the number of microglia in the miR-96-overexpressing retinas was significantly increased. Our findings demonstrate that appropriate miR-96 expression is required for mouse retinal homeostasis.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Degeneración Retiniana
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Microglía
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MicroARNs
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Ratones Endogámicos C57BL
Límite:
Animals
Idioma:
En
Año:
2024
Tipo del documento:
Article