In vitro developmental competence of bovine demi-embryos generated by blastomere separation and blastocyst bisection.
Reprod Domest Anim
; 59(6): e14627, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38837827
ABSTRACT
The efficiency of bovine in vitro embryo production can be significantly improved by splitting embryos at different stages. However, the blastocyst quality of in vitro-produced demi-embryos remains unexplored. The objective of this research was to compare embryo developmental rates and quality of bovine demi-embryos produced by two different strategies (a) embryo bisection (BSEC) and (b) 2-cell blastomere separation (BSEP). To determine demi-embryos quality, we evaluated total blastocyst cell number and proportion of SOX2+ cells. Additionally, the expression of SOX2, NANOG, OCT4, CDX2, IFNT, BAX and BCL genes and let-7a and miRNA-30c Micro RNAs was analysed. BSEP resulted in improved blastocyst development, higher ICM cells and a significantly higher expression of IFNΤ than demi-embryos produced by BSEC. Let-7a, which is associated with low pregnancy establishment was detected in BSEC, while miRNA-30c expression was observed in all treatments. In conclusion, BSEP of 2-cell embryos is more efficient to improve in vitro bovine embryo development and to produce good quality demi-embryos based on ICM cell number and the expression pattern of the genes explored compared to BSEC.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Blastocisto
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Blastómeros
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Técnicas de Cultivo de Embriones
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Desarrollo Embrionario
Límite:
Animals
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Pregnancy
Idioma:
En
Año:
2024
Tipo del documento:
Article