Regulation of candidalysin underlies Candida albicans persistence in intravascular catheters by modulating NETosis.
PLoS Pathog
; 20(6): e1012319, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38885290
ABSTRACT
Candida albicans is a leading cause of intravascular catheter-related infections. The capacity for biofilm formation has been proposed to contribute to the persistence of this fungal pathogen on catheter surfaces. While efforts have been devoted to identifying microbial factors that modulate C. albicans biofilm formation in vitro, our understanding of the host factors that may shape C. albicans persistence in intravascular catheters is lacking. Here, we used multiphoton microscopy to characterize biofilms in intravascular catheters removed from candidiasis patients. We demonstrated that, NETosis, a type of neutrophil cell death with antimicrobial activity, was implicated in the interaction of immune cells with C. albicans in the catheters. The catheter isolates exhibited reduced filamentation and candidalysin gene expression, specifically in the total parenteral nutrition culture environment. Furthermore, we showed that the ablation of candidalysin expression in C. albicans reduced NETosis and conferred resistance to neutrophil-mediated fungal biofilm elimination. Our findings illustrate the role of neutrophil NETosis in modulating C. albicans biofilm persistence in an intravascular catheter, highlighting that C. albicans can benefit from reduced virulence expression to promote its persistence in an intravascular catheter.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Candida albicans
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Candidiasis
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Proteínas Fúngicas
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Biopelículas
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Infecciones Relacionadas con Catéteres
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Trampas Extracelulares
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Neutrófilos
Límite:
Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article