The ethylene-responsive transcription factor ERF024 is a novel regulator of climacteric fruit ripening in melon.
Plant J
; 119(4): 1844-1858, 2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-38900073
ABSTRACT
Fruit ripening is an essential developmental stage in Angiosperms triggered by hormonal signals such as ethylene, a major player in climacteric ripening. Melon is a unique crop showing both climacteric and non-climacteric cultivars, offering an ideal model for dissecting the genetic mechanisms underpinning this process. The major quantitative trait locus ETHQV8.1 was previously identified as a key regulator of melon fruit ripening. Here, we narrowed down ETHQV8.1 to a precise genomic region containing a single gene, the transcription factor CmERF024. Functional validation using CRISPR/Cas9 knock-out plants unequivocally identified CmERF024 as the causal gene governing ETHQV8.1. The erf024 mutants exhibited suppression of ethylene production, leading to a significant delay and attenuation of fruit ripening. Integrative multi-omic analyses encompassing RNA-seq, DAP-seq, and DNase-seq revealed the association of CmERF024 with chromatin accessibility and gene expression dynamics throughout fruit ripening. Our data suggest CmERF024 as a novel regulator of climacteric fruit ripening in melon.
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1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
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Factores de Transcripción
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Regulación de la Expresión Génica de las Plantas
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Cucurbitaceae
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Etilenos
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Frutas
Idioma:
En
Año:
2024
Tipo del documento:
Article