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Chemical Resolution of an Epitope Recognized by Blood Group Antibodies Capable of Binding Both A and B Red Blood Cells.
Obukhova, Polina; Tyrtysh, Tatiana; Tsygankova, Svetlana; Paramonov, Alexander; Gordeeva, Elena; Shilova, Nadezhda; Lipatnikov, Alexander; Sokolova, Maria; Henry, Stephen; Salimov, Emin; Bovin, Nicolai; Ryzhov, Ivan.
  • Obukhova P; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Laboratory of carbohydrates, RUSSIAN FEDERATION.
  • Tyrtysh T; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Laboratory of carbohydrates, RUSSIAN FEDERATION.
  • Tsygankova S; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Laboratory of carbohydrates, RUSSIAN FEDERATION.
  • Paramonov A; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Laboratory of NMR, RUSSIAN FEDERATION.
  • Gordeeva E; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Laboratory of chemistry of proteolytic enzymes, RUSSIAN FEDERATION.
  • Shilova N; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Laboratory of carbohydrates, RUSSIAN FEDERATION.
  • Lipatnikov A; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Laboratory of carbohydrates, RUSSIAN FEDERATION.
  • Sokolova M; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Laboratory of carbohydrates, RUSSIAN FEDERATION.
  • Henry S; Auckland University of Technology, KODE, NEW ZEALAND.
  • Salimov E; I M Sechenov First Moscow State Medical University Scientific-Research Institute of Public Health and Health Management, Center of blood transfusion, RUSSIAN FEDERATION.
  • Bovin N; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences, Laboratory of carbohydrates, RUSSIAN FEDERATION.
  • Ryzhov I; Institution of bioorganic chemsistry RAS, Laboratory of carbohydrates, Miklukho-Maklaya, 16/10, 117997, Moscow, RUSSIAN FEDERATION.
Chembiochem ; : e202400430, 2024 Jun 20.
Article en En | MEDLINE | ID: mdl-38900551
ABSTRACT
The high specificity of human antibodies to blood group A and B antigens is impressive, especially when considering the structural difference between these antigens (tetrasaccharides) is a NHAc versus a OH-group on the terminal monosaccharide residue. It is well established that in addition to anti-A and anti-B there is a third antibody, anti-A,B capable of recognizing both A and B antigens. To analyze this AB specificity, we synthesized a tetrasaccharide, where the NHAc of the A antigen was replaced with NH2. This NH2-group was used to attach the glycan to an affinity resin, creating an AB-epitope (ABep) adsorbent where the critical site for recognition by A and B antibodies was not accessible, while the rest of the (conformationally compact) tetrasaccharide remained accessible. Anti-ABep antibodies were isolated from blood group O donors and found to have expected A,B-specificity against immobilized and red cell bound synthetic antigens, including ABep, and were able to agglutinate both A and B red cells. The amount of these anti-ABep (anti-A,B) antibodies found in the blood of group O donors was comparable to levels of anti-A and anti-B found in group B and A individuals. Using STD-NMR the location for the AB-epitope on the tetrasaccharide was found.
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2024 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Idioma: En Año: 2024 Tipo del documento: Article