Polyvinylamine and Its Derivative as Effective Carrier for Targeted Delivery of Small RNAs.
Methods Mol Biol
; 2822: 353-365, 2024.
Article
en En
| MEDLINE
| ID: mdl-38907928
ABSTRACT
Polymeric delivery systems could enable the fast- and low-side-effect transport of various RNA classes. Previously, we demonstrated that polyvinylamine (PVAm), a cationic polymer, transfects many kinds of RNAs with high efficiency and low toxicity both in vitro and in vivo. The modification of poly lactic-co-glycolic acid (PLGA) with cartilage-targeting peptide (CAP) enhances its stiffness and tissue-specific delivery of RNA to overcome the avascular nature of articular cartilage. Here we describe the protocol to use PVAm as an RNA carrier, and further, by modifying PVAm with PLGA and CAP, the corresponding co-polymer could be applied for functional RNA delivery for osteoarthritis treatment.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polivinilos
/
Copolímero de Ácido Poliláctico-Ácido Poliglicólico
Límite:
Animals
/
Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article