Protocol for long-term monocultures of murine macrophages derived from distinct adult tissues.
STAR Protoc
; 5(3): 103145, 2024 Jun 22.
Article
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| MEDLINE
| ID: mdl-38909360
ABSTRACT
Tissue-resident macrophages (TRMs) constitute the first line of defense against infection in all organs and perform organ-specific functions during tissue homeostasis. Here, we present a protocol for long-term monocultures of murine macrophages from different adult organs, including the brain, liver, peritoneal cavity, and lung. We describe steps for tissue preparation and the use of a combination of organotypic conditions to maintain a TRM-like identity in vitro, resulting in an ideal screening platform for a wide range of assays and readouts. For complete details on the use and execution of this protocol, please refer to Aktories et al.1.
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2024
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