MAST4 regulates stem cell maintenance with DLX3 for epithelial development and amelogenesis.
Exp Mol Med
; 56(7): 1606-1619, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38945953
ABSTRACT
The asymmetric division of stem cells permits the maintenance of the cell population and differentiation for harmonious progress. Developing mouse incisors allows inspection of the role of the stem cell niche to provide specific insights into essential developmental phases. Microtubule-associated serine/threonine kinase family member 4 (Mast4) knockout (KO) mice showed abnormal incisor development with low hardness, as the size of the apical bud was decreased and preameloblasts were shifted to the apical side, resulting in amelogenesis imperfecta. In addition, Mast4 KO incisors showed abnormal enamel maturation, and stem cell maintenance was inhibited as amelogenesis was accelerated with Wnt signal downregulation. Distal-Less Homeobox 3 (DLX3), a critical factor in tooth amelogenesis, is considered to be responsible for the development of amelogenesis imperfecta in humans. MAST4 directly binds to DLX3 and induces phosphorylation at three residues within the nuclear localization site (NLS) that promotes the nuclear translocation of DLX3. MAST4-mediated phosphorylation of DLX3 ultimately controls the transcription of DLX3 target genes, which are carbonic anhydrase and ion transporter genes involved in the pH regulation process during ameloblast maturation. Taken together, our data reveal a novel role for MAST4 as a critical regulator of the entire amelogenesis process through its control of Wnt signaling and DLX3 transcriptional activity.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Células Madre
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Factores de Transcripción
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Ratones Noqueados
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Proteínas de Homeodominio
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Amelogénesis
Límite:
Animals
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Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article