Mechanical forces remodel the cardiac extracellular matrix during zebrafish development.
Development
; 151(13)2024 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-38984541
ABSTRACT
The cardiac extracellular matrix (cECM) is fundamental for organ morphogenesis and maturation, during which time it undergoes remodeling, yet little is known about whether mechanical forces generated by the heartbeat regulate this remodeling process. Using zebrafish as a model and focusing on stages when cardiac valves and trabeculae form, we found that altering cardiac contraction impairs cECM remodeling. Longitudinal volumetric quantifications in wild-type animals revealed region-specific dynamics cECM volume decreases in the atrium but not in the ventricle or atrioventricular canal. Reducing cardiac contraction resulted in opposite effects on the ventricular and atrial ECM, whereas increasing the heart rate affected the ventricular ECM but had no effect on the atrial ECM, together indicating that mechanical forces regulate the cECM in a chamber-specific manner. Among the ECM remodelers highly expressed during cardiac morphogenesis, we found one that was upregulated in non-contractile hearts, namely tissue inhibitor of matrix metalloproteinase 2 (timp2). Loss- and gain-of-function analyses of timp2 revealed its crucial role in cECM remodeling. Altogether, our results indicate that mechanical forces control cECM remodeling in part through timp2 downregulation.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pez Cebra
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Inhibidor Tisular de Metaloproteinasa-2
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Matriz Extracelular
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Corazón
Límite:
Animals
Idioma:
En
Año:
2024
Tipo del documento:
Article