Ex Vivo Fluorescent Labeling of HBV-Specific B Cells in Chronic Hepatitis B Patients.
Methods Mol Biol
; 2837: 241-255, 2024.
Article
en En
| MEDLINE
| ID: mdl-39044090
ABSTRACT
Fluorescently conjugated antigen-bait systems have been extensively used to identify antigen-specific B cells and probe humoral immunity across different settings. Following this approach, we used HBV antigens to bind the B cell receptor (BCR), permitting antigen-specific B cell detection by flow cytometry. Fluorochromes can either be attached covalently via chemical conjugation to the antigen or attached non-covalently by biotinylating the antigen. Dual-staining antigen-baits (where an antigen is directly conjugated to two distinct fluorochromes) have now been used to identify HBsAg- and HBcAg-specific B cells with a high degree of reliability and specificity. This system can be used to detect and characterize cells ex vivo or adapted to isolate antigen-specific cells using fluorescence-activated cell sorting.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos B
/
Virus de la Hepatitis B
/
Hepatitis B Crónica
/
Citometría de Flujo
/
Colorantes Fluorescentes
/
Antígenos de Superficie de la Hepatitis B
Límite:
Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article