Adiponectin receptor 1 regulates endometrial receptivity via the adenosine monophosphateactivated protein kinase/Ecadherin pathway.
Mol Med Rep
; 30(4)2024 Oct.
Article
en En
| MEDLINE
| ID: mdl-39155876
ABSTRACT
Endometrial receptivity is essential for successful embryo implantation and pregnancy initiation and is regulated via various signaling pathways. Adiponectin, an important adipokine, may be a potential regulator of reproductive system functions. The aim of the present study was to elucidate the regulatory role of adiponectin receptor 1 (ADIPOR1) in endometrial receptivity. The endometrial receptivity between RL952 and AN3CA cell lines was confirmed using an in vitro JAr spheroid attachment model. 293T cells were transfected with control or short hairpin (sh)ADIPOR1 vectors and RL952 cells were transduced with lentiviral particles targeting ADIPOR1. Reverse transcriptionquantitative PCR and immunoblot assays were also performed. ADIPOR1 was consistently upregulated in the endometrium during the midsecretory phase compared with that in the proliferative phase and in receptive RL952 cells compared with that in nonreceptive AN3CA cells. Stable cell lines with diminished ADIPOR1 expression caused by shRNA showed reduced Ecadherin expression and attenuated in vitro endometrial receptivity. ADIPOR1 regulated AMPactivated protein kinase (AMPK) activity in endometrial epithelial cells. Regulation of AMPK activity via dorsomorphin and 5aminoimidazole4carboxamide ribonucleotide affected Ecadherin expression and in vitro endometrial receptivity. The ADIPOR1/AMPK/Ecadherin axis is vital to endometrial receptivity. These findings can help improve fertility treatments and outcomes.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Transducción de Señal
/
Cadherinas
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Endometrio
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Receptores de Adiponectina
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Proteínas Quinasas Activadas por AMP
Límite:
Adult
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Female
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Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article