Improving normothermic machine perfusion and blood transfusion through biocompatible blood silicification.
Proc Natl Acad Sci U S A
; 121(35): e2322418121, 2024 Aug 27.
Article
en En
| MEDLINE
| ID: mdl-39159377
ABSTRACT
The growing world population and increasing life expectancy are driving the need to improve the quality of blood transfusion, organ transplantation, and preservation. Here, to improve the ability of red blood cells (RBCs) for normothermic machine perfusion, a biocompatible blood silicification approach termed "shielding-augmenting RBC-in-nanoscale amorphous silica (SARNAS)" has been developed. The key to RBC surface engineering and structure augmentation is the precise control of the hydrolysis form of silicic acid to realize stabilization of RBC within conformal nanoscale silica-based exoskeletons. The formed silicified RBCs (Si-RBCs) maintain membrane/structural integrity, normal cellular functions (e.g., metabolism, oxygen-carrying capability), and enhance resistance to external stressors as well as tunable mechanical properties, resulting in nearly 100% RBC cryoprotection. In vivo experiments confirm their excellent biocompatibility. By shielding RBC surface antigens, the Si-RBCs provide universal blood compatibility, the ability for allogeneic mechanical perfusion, and more importantly, the possibility for cross-species transfusion. Being simple, reliable, and easily scalable, the SARNAS strategy holds great promise to revolutionize the use of engineered blood for future clinical applications.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Materiales Biocompatibles
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Dióxido de Silicio
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Eritrocitos
Límite:
Animals
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Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article