A single-molecule fluorescent probe for visualizing viscosity and hypoxia in lysosomes and zebrafish embryos.
Analyst
; 149(19): 4946-4952, 2024 Sep 23.
Article
en En
| MEDLINE
| ID: mdl-39171959
ABSTRACT
Viscosity and hypoxia, as microenvironment parameters, play important roles in maintaining normal biological processes and homeostasis. Therefore, simultaneous and sensitive detection of these elements with simple and effective methods could offer precise information in biology. Here, we report a two-site lysosome-targeting fluorescent probe, NVP, for monitoring viscosity and nitroreductase with dual emission channels (emission shift is 86 nm). The NVP probe has displayed highly sensitive and selective responses towards viscosity and nitroreductase, respectively. Significantly, the fluctuations of viscosity and NTR have been detected in vitro and in vivo. We expect that the dual-responsive fluorescent NVP probe will become a potential molecular tool for the exploration of deeper functions of viscosity and hypoxia.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pez Cebra
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Embrión no Mamífero
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Colorantes Fluorescentes
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Lisosomas
Límite:
Animals
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Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article