Roe deer exposure to trace metals and pesticides in forests and agricultural plains of North-eastern France.

ABSTRACT

Human activities related to agriculture and industrial development result in the emission of different types of pollutants in the environment. The phytosanitary pressure depends on the time of the year, the type of habitat, and the used treatments. Wildlife, particularly ungulates, can be exposed to pesticides and trace metals through their herbivorous diet directly impregnated by the environmental contamination. Wild game thus plays a sentinel role but can also represent a risk for human health by exposing venison consumers to these contaminants. The present study explored this dual problem in two types of habitat forests and agricultural plains located in North-eastern France. Samples of liver and muscle were taken from ten roe deer, five caught on the forests and five others in the plains, to determine contaminant levels. There was no significant difference in contamination of roe deer livers according to the habitat for three trace metals (chromium, nickel, and copper), while lead concentrations were higher in samples from the plain (0.85 vs. 0.74 mg·kg-1 dry matter DM; P < 0.01). Conversely, zinc concentrations were higher in forest roe deer (110 vs. 95 mg·kg-1 DM; P < 0.05). Cadmium concentrations did not differ significantly between the two habitats, but forest samples showed strong inter-individual variability, with some values close to those observed on the plains and others higher (1.8 vs. 0.3 mg·kg-1 DM for forest and plain, respectively; P = 0.11). Mercury was not detected in any sample. The majority of pesticides investigated were not detected. Only a few pesticides were in trace amount (from 1 to 8 µg·kg-1 fresh matter), either in the liver (e.g., heptachlor and oxychlordane) or in both tissues, liver and muscle (e.g., pyridalyl). Some compounds were only detected in plain (e.g., cypermethrin and fenpropidin) and others in forest (piperonyl butoxide and pyridalyl) samples. Analyses mainly detected insecticides in samples taken from forests and agricultural plains. Fungicides and a few herbicides were mainly detected in plain sampled animals. Pesticides and copper concentrations in roe deer tissues were lower than maximum residue levels established for farm (copper) or wild terrestrial (pesticides) animals. Cadmium concentrations were also lower than the maximum level for most samples, except for some forest roe deer. However, both forest and plain roe deer exceeded the maximum level for lead in liver. Human exposure was simulated according to different amounts of meat consumed and compared with toxicological reference values. This study highlighted an overall low risk to human health for both pesticides, copper and lead, with more reservations about cadmium for some roe deer shot in forest. The differences in roe deer contamination between forests and agricultural plains for some trace elements and organic pollutants confirmed the sentinel role of this species.