Nr2f1 enhancers have distinct functions in controlling Nr2f1 expression during cortical development.
Proc Natl Acad Sci U S A
; 121(40): e2402368121, 2024 Oct.
Article
en En
| MEDLINE
| ID: mdl-39312666
ABSTRACT
There is evidence that transcription factor (TF) encoding genes, which temporally control development in multiple cell types, can have tens of enhancers that regulate their expression. The NR2F1 TF developmentally promotes caudal and ventral cortical regional fates. Here, we epigenomically compared the activity of Nr2f1's enhancers during mouse cortical development with their activity in a transgenic assay. We identified at least six that are likely to be important in prenatal cortical development, with three harboring de novo mutants identified in ASD individuals. We chose to study the function of two of the most robust enhancers by deleting them singly or together. We found that they have distinct and overlapping functions in driving Nr2f1's regional and laminar expression in the developing cortex. Thus, these two enhancers, probably in combination with the others that we defined epigenetically, precisely tune Nr2f1's regional, cell type, and temporal expression during corticogenesis.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Corteza Cerebral
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Elementos de Facilitación Genéticos
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Regulación del Desarrollo de la Expresión Génica
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Factor de Transcripción COUP I
Límite:
Animals
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Female
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Humans
Idioma:
En
Año:
2024
Tipo del documento:
Article