A specific radioimmunoassay for human beta-lipotropin.
J Clin Endocrinol Metab
; 47(1): 160-7, 1978 Jul.
Article
en En
| MEDLINE
| ID: mdl-400711
ABSTRACT
A homologous RIA for human beta-lipotropin (beta hLPH) has been developed. At a final dilution of 124,000, the antiserum employed shows cross-reaction with beta hLPH but none with human beta-MSH (beta hMSH), and it is concluded that the antigenic determinant lies within the N-terminal 1-36 region of beta hLPH. With extraction of 3-ml plasma samples, the assay is sufficiently sensitive to measure circulating beta hLPH levels in normal individuals at 0900 h (25-200 pg/ml). There is a circadian variation with levels falling to (less than 20-80 pg/ml) at 2300 h. beta hLPH levels rise after metyrapone and after insulin-induced hypoglycemia, and fall after administration of dexamethasone. In patients with a variety of diseases of the pituitary-adrenal axis, levels of beta hLPH follow immunoreactive ACTH levels, although the two are not always secreted on a 11 molar basis.
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Banco de datos:
MEDLINE
Asunto principal:
Beta-Lipotropina
Límite:
Humans
Idioma:
En
Año:
1978
Tipo del documento:
Article