Replication of adenovirus type 7 in monkey cells: a new determinant and its transfer to adenovirus type 2.
Science
; 154(3749): 671-3, 1966 Nov 04.
Article
en En
| MEDLINE
| ID: mdl-4958853
ABSTRACT
A strain of human adenovirus type 7, adapted to replication in green-monkey kidney cells, requires the interaction of two particles to initiate plaque formation in the simian cells. One particle is a true adenovirion. The second, apparently defective, consists of a genome carrying amonkey-adapting component in an adenovirus capsid; this genome does not express known SV40 determinants. The addition of human adenovirus type 7 that is not adapted enhances the titer and changesconditions for plaque formation by the adapted virus to a one-particle requirement. Addition of nonadapted human adenovirus type 2 as helper virus results in the transfer of the monkey-adaptingcomponent from adenovirus type 7 to adenovirus type 2. The population containing the adenovirus 2 transcapsidant then has the ability to replicate in simian cells.
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Banco de datos:
MEDLINE
Asunto principal:
Cultivo de Virus
/
Adenoviridae
/
Riñón
Límite:
Animals
Idioma:
En
Año:
1966
Tipo del documento:
Article