5-Azacytidine-induced reactivation of a herpes simplex thymidine kinase gene.
Science
; 216(4541): 70-3, 1982 Apr 02.
Article
en En
| MEDLINE
| ID: mdl-6175023
ABSTRACT
Mouse cells transformed with herpes simplex virus and containing the viral thymidine kinase (TK) gene in an inactive state were treated with 5-azacytidine. The result was the reexpression of the viral TK gene. Two days of exposure to 5-azacytidine followed by 2 days of expression time was sufficient for maximal induction of the TK+ phenotype. The induction of TK expression by 5-azacytidine was concentration-dependent, with maximal induction at 10 micromoles per liter. 5-Azacytidine also inhibited the decay of TK expression in TK+ transformants removed from selective conditions. Analysis of the methylation patterns of the viral TK gene with restriction endonucleases Hpa II and Msp I showed the active gene to be unmethylated, the inactive gene methylated, and the 5-azacytidine-induced gene unmethylated.
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Banco de datos:
MEDLINE
Asunto principal:
Azacitidina
/
Timidina Quinasa
/
Transformación Celular Viral
/
Regulación de la Expresión Génica
/
Simplexvirus
Idioma:
En
Año:
1982
Tipo del documento:
Article