Variability in DNA sequence of closely related nosocomial gentamicin-resistant plasmids.
J Infect Dis
; 148(6): 1013-8, 1983 Dec.
Article
en En
| MEDLINE
| ID: mdl-6317765
ABSTRACT
Endemic gentamicin-resistant plasmids derived from 12 species of gram-negative bacilli were analyzed by restriction-endonuclease digestion. These digests showed wide variations in digest patterns, but one or more common fragments were seen in all plasmids studied. Southern blot analysis using one plasmid as a probe revealed that most but not all of these plasmids shared extensive regions of homology. The variance observed in restriction-digest patterns could be accounted for either by introduction of unrelated DNA or by intramolecular events. We conclude that variation in restriction-digest patterns of endemic gentamicin-resistant plasmids is insufficient to establish that the plasmids are not closely related. Variation in molecular structure of closely related, endemic gentamicin-resistant plasmids may be more common than has previously been suspected.
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Banco de datos:
MEDLINE
Asunto principal:
Pseudomonas
/
ADN Bacteriano
/
Factores R
/
Gentamicinas
/
Desoxirribonucleasas de Localización Especificada Tipo II
/
Enterobacteriaceae
Idioma:
En
Año:
1983
Tipo del documento:
Article