Factor VIII-related antigen in occluded human arteries and grafts.

ABSTRACT

Direct immunofluorescence was used to determine the disposition of factor VIII-related antigen (VIIIRAg) in occluded arteries and grafts from patients undergoing reconstructive operation. The presence of VIIIRAg on the luminal surface of these vessels was equated with their endothelialization according to the work of others. In 12 of the 20 arteries examined, stain for VIIIRAg was absent or markedly reduced from the luminal surfaces, and in six more it was only present focally. The adventitial capillaries were brightly stained in most of these specimens. Both of two saphenous vein grafts and all of six Dacron grafts stained for VIIIRAg along their luminal surfaces and in the adventitial capillaries as brightly as normal arteries. However, all of eight Gore-tex grafts had little or no stain for VIIIRAg along their luminal surfaces, and the capillaries of the adventitia were not as plentiful as those in the normal arteries and Dacron grafts. With one exception, the Gore-tex grafts had become occluded less than 1 year after implantation, while the Dacron grafts had remained patent for 2 to 8 years. Thus many of the diseased arteries and the short-lived Gore-tex grafts were characterized by relative absence of VIIIRAg from their luminal surfaces, presumably reflecting the loss or absence of endothelialization.