Functional maturation of B cells in vitro.
Immunology
; 32(3): 275-81, 1977 Mar.
Article
en En
| MEDLINE
| ID: mdl-66196
ABSTRACT
Maturation of B-cell function was studied in a two-stage tissue culture system. In the first stage, cells were cultured in the absence of antigen and then transferred to microcultures where the frequency of hapten-specific plaque-forming cell (PFC) precursors was determined; Bone-marrow cells and spleen cells from 6--8-day-old mice mice were shown to act as sources of B-cell neogenesis in vitro. Both populations had very low initial frequencies of hapten-specific PFC precursors, but this increased ten- to seventeen-fold during a period of 72 h in the preliminary cultures. This increase could not be accounted for by selective cell death, nor by decay of a suppressor cell subpopulation nor by proliferation of pre-existing Fc-receptor-bearing B cells. The mechanism for the increase in frequency of functional B cells in cultures of bone marrow and neonatal spleen was thus the result of maturation of B-cell precursors to a state of immune competence during the culture interval.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos B
/
Formación de Anticuerpos
Límite:
Animals
Idioma:
En
Año:
1977
Tipo del documento:
Article