Studies on lipid A from Yersinia pseudotuberculosis lipopolysaccharide. Isolation and general characterization.
Eur J Biochem
; 89(1): 287-9, 1978 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-699914
ABSTRACT
Lipid A was isolated from lipopolysaccharide of Yersinia pseudotuberculosis S form (strain 341, subtype IB) using mild hydrolysis with acetic acid. The purified material (yield about 25%, molecular weight about 2900) contained D-glucosamine (11%), fatty acids (54%), protein concomitant (9.7%) and phosphorus (approximately 2%). Dodecanoic and 3-hydroxy-tetradecanoic acids in a molar ratio of 1 3.6 were detected as major fatty acid constituents. The hydroxyl groups of D-glucosamine were acylated with the residues of both fatty acids, while the amino groups were substituted with the residue of 3-hydroxy-tetradecanoic acid. Such a simple fatty acid composition is reminiscent of that found in lipid A in Y. pestis.
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Banco de datos:
MEDLINE
Asunto principal:
Yersinia
/
Lipopolisacáridos
/
Lípido A
Idioma:
En
Año:
1978
Tipo del documento:
Article