High performance reversed phase chromatography of cholesterol and cholesteryl esters of human plasma lipoproteins.
Lipids
; 16(8): 609-13, 1981 Aug.
Article
en En
| MEDLINE
| ID: mdl-7278520
ABSTRACT
Cholesterol and cholesteryl esters were separated according to their carbon number and number of double bonds by high performance reversed-phase chromatography (HPRC) using acetonitrile/chloroform/methanol (111, v/v) as a mobile phase. It was found that within the same equivalent carbon number (ECN) category, cholesteryl esters with the highest number of double bonds eluted ahead of those with a lower number of double bonds, and with the cis isomers eluting ahead of their trans partners. Thus, cholesteryl oleate (C27-181c) elutes ahead of cholesteryl palmitate (C27-160) and ahead of cholesteryl elaidate (C27-181t). Human lipoprotein, as well as rat liver cholesteryl esters, were separated using this technique.
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Banco de datos:
MEDLINE
Asunto principal:
Colesterol
/
Ésteres del Colesterol
/
Lipoproteínas
Límite:
Humans
Idioma:
En
Año:
1981
Tipo del documento:
Article