Purification and properties of a novel enzyme from Bacillus spp. T-3040, which catalyzes the conversion of dextran to cyclic isomaltooligosaccharides.
FEBS Lett
; 345(2-3): 135-8, 1994 May 30.
Article
en En
| MEDLINE
| ID: mdl-7515357
ABSTRACT
A novel enzyme, cycloisomaltooligosaccharide glucanotransferase (CITase), catalyzes the conversion of dextran to cyclic isomaltooligosaccharides by intramolecular transglucosylation (cyclization reaction). CITase was purified to homogeneity from the culture filtrate of Bacillus sp. T-3040 isolated from soil. The Mr of the enzyme was estimated to be 98,000 by SDS-PAGE. The enzyme catalyzed the cyclization reaction and gave three cyclic isomaltooligosaccharides (cycloisomalto-heptaose, -octase, and -nonaose) at a total yield of about 20%. Coupling and disproportionation reactions were also observed. These results showed that this enzyme is a multi-functional enzyme which catalyzes intramolecular and intermolecular transglucosylation.
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Banco de datos:
MEDLINE
Asunto principal:
Oligosacáridos
/
Bacillus
/
Dextranos
/
Glucosiltransferasas
Idioma:
En
Año:
1994
Tipo del documento:
Article