Role of major histocompatibility complex class I expression and natural killer-like T cells in the genetic control of endometriosis.
Fertil Steril
; 64(5): 909-16, 1995 Nov.
Article
en En
| MEDLINE
| ID: mdl-7589633
ABSTRACT
OBJECTIVE:
To evaluate whether the expression of human leukocyte antigen (HLA) class I on eutopic and ectopic endometrial cells modify the susceptibility to lysis mediated by lymphocytes.DESIGN:
Evaluation of T lymphocyte cytotoxic activity and HLA class I expression on endometrial cells.SETTING:
Subjects were recruited at laparoscopy. PATIENTS Patients with endometriosis (n = 7). Healthy women as controls (n = 10). MAIN OUTCOMEMEASURES:
Human leukocyte antigen class I molecule analysis of endometrial cells was carried out by immunofluorescence and flow cytometry. Phenotyping of T lymphocytes was performed to analyze T-cell subsets. Cytotoxicity was performed to determine cytolytic activity against endometrial cells.RESULTS:
In vitro culture of endometrial cells down-regulates the expression of HLA class I molecules and enhances the susceptibility to lysis mediated by natural killer (NK)-like T lymphocytes. Cytolytic T-cell clones, expressing the CD94 antigen, are inhibited by the HLA-B7 allele on endometrial cells. Ectopic endometrial cells modulate the expression of HLA class I molecules.CONCLUSIONS:
The resistance to lysis of endometrial cells is related to expression of surface HLA class I molecules, which send a negative signal for lysis mediated by NK-like T lymphocytes. The HLA-B7 allele inhibits the cytotoxic activity, suggesting that the growth of ectopic endometrial cells might be under a genetic control.
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Banco de datos:
MEDLINE
Asunto principal:
Células Asesinas Naturales
/
Antígenos de Histocompatibilidad Clase I
/
Endometriosis
Tipo de estudio:
Etiology_studies
Límite:
Female
/
Humans
Idioma:
En
Año:
1995
Tipo del documento:
Article