Reversal of Mu gem2ts-induced mutations.
FEMS Microbiol Rev
; 17(1-2): 171-6, 1995 Aug.
Article
en En
| MEDLINE
| ID: mdl-7669343
ABSTRACT
Mutations induced by the integration of a Mu gem2ts mutant prophage can revert at frequencies around 1 x 10(-6), more than 10(4)-fold higher than that obtained with Mu wild-type. Several aspects characterize Mu gem2ts precise excision (i) the phage transposase is not involved; (ii) the RecA protein is not necessary; and (iii) revertants remain lysogenic with the prophage inserted elsewhere in the host genome. In addition, prophage re-integration seems to be non-randomly distributed, whereas Mu insertion into the host genome is a transposition event without any sequence specificity. In this paper, we describe that the site of re-integration somehow depends on the original site of insertion. Two alternative models are proposed to explain the strong correlation between donor and receptor sites.
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Banco de datos:
MEDLINE
Asunto principal:
Provirus
/
Bacteriófago mu
/
Mutación
Idioma:
En
Año:
1995
Tipo del documento:
Article