2-LTR circular viral DNA as a marker for human immunodeficiency virus type 1 infection in vivo.
Virology
; 205(2): 470-8, 1994 Dec.
Article
en En
| MEDLINE
| ID: mdl-7975248
ABSTRACT
The unintegrated viral DNA found in human immunodeficiency virus type 1 infection includes linear and circular forms. We targeted the circular form containing two copies of the viral long terminal repeat (2-LTR circle) and developed specific assays to detect this molecule in peripheral blood mononuclear cells from HIV-infected patients. In vitro HIV-1 infection of peripheral blood mononuclear cells showed rapid accumulation and rapid decay of 2-LTR circular viral DNA. Examination of 2-LTR circular viral DNA levels provides a view of spreading infection based on a viral DNA form that is structurally distinct and has a known, short half-life in infected cells. In patients not receiving antiviral therapy, the levels of 2-LTR circular viral DNA and total viral DNA were significantly correlated to CD4 cell counts. Similar correlations were not observed in patients receiving zidovudine (AZT), didanosine (ddA), or zalcitabine (ddC).
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Banco de datos:
MEDLINE
Asunto principal:
ADN Circular
/
ADN Viral
/
Infecciones por VIH
/
Duplicado del Terminal Largo de VIH
/
VIH-1
Límite:
Humans
Idioma:
En
Año:
1994
Tipo del documento:
Article