Effects of glucose, ethanol, Hg(II) and Cu(II) on almond beta-glucosidase.
Biochem Mol Biol Int
; 33(5): 939-46, 1994 Aug.
Article
en En
| MEDLINE
| ID: mdl-7987262
ABSTRACT
The kinetic parameters of almond beta-glucosidase (beta-D-glucoside glucohydrolase; EC 3.2.1.21), using pNGP as substrate were kM = 2.24 +/- 0.11 mM and Vmax 588 +/- 25.1 U/mg protein. Only Hg(II) and Cu(II) showed irreversible inactivation of the enzyme. However, when these metals were present in the reaction system the inhibition effects were consistent with a mixed-type inhibition pattern (Cu(II) ki 5.08 mM and Hg(II) ki 0.07 mM). The glucose kinetic effect was also consistent with a mixed-type inhibition (ki = 406 mM) pattern with pNGP as varied substrate. Ethanol displayed the kinetic pattern of competitive inhibition (ki = 640 mM).
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Banco de datos:
MEDLINE
Asunto principal:
Beta-Glucosidasa
/
Nueces
Idioma:
En
Año:
1994
Tipo del documento:
Article