Functional expression of human renal Na+/Ca2+ exchanger in insect cells.
Am J Physiol
; 267(1 Pt 2): F70-4, 1994 Jul.
Article
en En
| MEDLINE
| ID: mdl-8048567
ABSTRACT
The cDNA coding for the human renal isoform of the Na+/Ca2+ exchanger was cloned from a human embryonic kidney cell line (HEK 293) using a polymerase chain reaction strategy. It was found by sequence analysis that the cDNA encoded for a human kidney isoform of the Na+/Ca2+ exchanger. The kidney isoform is nearly identical to the human cardiac Na+/Ca2+ exchanger except for a segment of amino acids within the predicted cytoplasmic loop region of the molecule. Within this region, the kidney isoform contains a deletion of 36 amino acids, as well as a segment of 33 amino acids that is only 33% identical to the equivalent region of the cardiac exchanger. The cDNA was then tested for function by expression in insect cells. We constructed a recombinant baculovirus containing renal Na+/Ca2+ exchanger cDNA under control of the polyhedrin promoter. High levels of expression and Na+/Ca2+ exchange activity were found in Sf9 insect cells infected with the recombinant virus. Our results indicate that human kidney expresses a functional and alternatively spliced variant of the human cardiac Na+/Ca2+ exchanger.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
Riñón
Límite:
Animals
/
Humans
Idioma:
En
Año:
1994
Tipo del documento:
Article