Ribozyme-mediated inhibition of bcr-abl gene expression in a Philadelphia chromosome-positive cell line.
Blood
; 82(2): 600-5, 1993 Jul 15.
Article
en En
| MEDLINE
| ID: mdl-8329715
ABSTRACT
The bcr-abl fusion gene is the molecular counterpart of the Philadelphia chromosome (Ph1) and is directly involved in the pathogenesis of Ph1+ leukemia. Inhibition of bcr-abl gene expression may have profound effects on the cell biology of Ph1+ cells, as recent experiments with antisense oligonucleotides have shown. In this study we have designed and synthesized a unique ribozyme that is directed against bcr-abl mRNA. The ribozyme cleaved bcr-abl mRNA in a cell-free in vitro system. A DNA-RNA hybrid ribozyme was then incorporated into a liposome vector and transfected into EM-2 cells, a cell line derived from a patient with blast crisis of chronic myelogenous leukemia. The ribozyme decreased levels of detectable bcr-abl mRNA in these cells, inhibited expression of the bcr-abl gene product, p210bcr-abl, and inhibited cell growth. This anti-bcr-abl ribozyme may be a useful tool to study the cell biology of Ph1+ leukemia and may ultimately have therapeutic potential in treating patients with Ph1 leukemias.
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Banco de datos:
MEDLINE
Asunto principal:
ARN Mensajero
/
Leucemia Mielógena Crónica BCR-ABL Positiva
/
Expresión Génica
/
ARN Catalítico
/
Proteínas de Fusión bcr-abl
Límite:
Humans
Idioma:
En
Año:
1993
Tipo del documento:
Article