The ZNF35 human zinc finger gene encodes a sequence-specific DNA-binding protein.
FEBS Lett
; 321(2-3): 233-6, 1993 Apr 26.
Article
en En
| MEDLINE
| ID: mdl-8477855
ABSTRACT
We developed a rapid method to determine DNA-binding sites for putative DNA-binding proteins. This procedure has been successfully used to define a specific consensus site for the human ZNF35 zinc finger gene. ZNF35 encodes a 58-kDA polypeptide containing 11 consecutive finger motifs located at the amino terminus, and an acidic domain located at the carboxy terminus. These features suggest that ZNF35 is a site-specific DNA-binding protein involved in the regulation of gene expression. We have expressed the ZNF35 protein from E. coli and have employed a Southwestern-polymerase chain reaction method using random oligonucleotides to identify its high-affinity binding site. The core sequence for the ZNF35 protein-binding site is 5'-C/GC/GAAG/TA-3'.
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Banco de datos:
MEDLINE
Asunto principal:
Dedos de Zinc
/
Proteínas de Unión al ADN
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
1993
Tipo del documento:
Article