Normal human chromosome 2 induces cellular senescence in the human cervical carcinoma cell line SiHa.
Genes Chromosomes Cancer
; 14(2): 120-7, 1995 Oct.
Article
en En
| MEDLINE
| ID: mdl-8527393
ABSTRACT
For identification of the chromosome carrying cellular senescence-inducing activity, normal human chromosome 2, 3, 6, 7, 9, 11, or 12 tagged with a selectable marker gene (neo) was introduced into the human cervical carcinoma cell line SiHa via microcell-mediated chromosome transfer. Seventy-six percent (158/207) of the G418-resistant clones obtained by the transfer of chromosome 2 showed a remarkable change in morphology (cells were flat), and 93% (147/158) of them ceased to divide (senesced) prior to 6-9 population doublings, whereas most of the clones generated by the transfer of other chromosomes exhibited a morphology similar to that of the parental cells and continued to grow. Chromosome analyses suggested that cells which escaped from senescence contained only a small fragment derived from the transferred chromosome 2, whereas the transferred chromosomes were apparently intact in most of the continuously growing microcell hybrids with introduction of other chromosomes. These results indicate that the normal human chromosome 2 carries a gene or genes that induce cellular senescence in SiHa cells.
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Banco de datos:
MEDLINE
Asunto principal:
Cromosomas Humanos Par 2
/
Carcinoma de Células Escamosas
/
Neoplasias del Cuello Uterino
/
Senescencia Celular
Límite:
Animals
/
Female
/
Humans
/
Middle aged
Idioma:
En
Año:
1995
Tipo del documento:
Article