Efficient expression, processing and secretion of a biologically active mammalian protein by Vibrio cholerae.
FEBS Lett
; 387(2-3): 167-70, 1996 Jun 03.
Article
en En
| MEDLINE
| ID: mdl-8674542
ABSTRACT
The use of Vibrio cholerae as a secretory expression system for the expression of a mammalian protein, namely human growth hormone, under the control of the heat labile enterotoxin chain B signal sequence is reported. The protein is efficiently expressed and processed. The mature protein is exported to the periplasm after which it is secreted to the extracellular milieu. The expressed and secreted hGH actively binds to its receptor as established by its receptor binding activity. The biological activity of the protein is demonstrated in vitro in a Nb2 proliferation assay.
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Banco de datos:
MEDLINE
Asunto principal:
Vibrio cholerae
/
Hormona del Crecimiento
Límite:
Animals
/
Humans
Idioma:
En
Año:
1996
Tipo del documento:
Article