Ligand-induced formation of hoogsteen-paired parallel DNA.
Chem Biol
; 3(1): 57-65, 1996 Jan.
Article
en En
| MEDLINE
| ID: mdl-8807829
ABSTRACT
INTRODUCTION:
Based on molecular modeling studies, a model has been proposed for intercalation of triple-helix-specific ligands (benzopyridoindole (BPI) derivatives) into triple helices, in which the intercalating compounds interact mainly with the Hoogsteen-paired strands of the triple helix. We set out to test this model experimentally using DNA duplexes capable of forming parallel Hoogsteen base-paired structures.RESULTS:
We have investigated the possible formation of a parallel DNA structure involving Hoogsteen hydrogen bonds by thermal denaturation, FTIR spectroscopy and gel-shift experiments. We show that BPI derivatives bind to Hoogsteen base-paired duplexes and stabilize them. The compounds induce a reorganization from a non-perfectly matched antiparallel Watson- Crick duplex into a perfectly matched parallel Hoogsteen-paired duplex.CONCLUSIONS:
These results suggest that preferential intercalation of BPI derivatives in triple helices is due to their ability to interact specifically with the Hoogsteen-paired bases. The results are consistent with a model proposed on the basis of molecular modeling studies using energy minimization, and they open a new field of investigations regarding the biological relevance of Hoogsteen base-pairing.
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Banco de datos:
MEDLINE
Asunto principal:
ADN
/
Conformación de Ácido Nucleico
Idioma:
En
Año:
1996
Tipo del documento:
Article